Device

Part:BBa_I766606

Designed by: Eric Chou   Group: iGEM07_UCSF   (2007-08-16)


YopH-EE under medium constitutive promotor

This device will express YopH, a pathogen derived reversible inhibitor (phosphotase) of Map Kinase Fus3 under the pCyc promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing a matching RR zipper. The effector is fused mCherry (RFP) on the N terminal.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 720
    Illegal PstI site found at 2035
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2035
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1675
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 720
    Illegal PstI site found at 2035
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 720
    Illegal PstI site found at 2035
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1225


[edit]
Categories
Parameters
None